vial
Reference: CAN-PRE-4500
Kit Type: Competitive ELISA
Kit Size: 96-well break-apart microplate
Sensitivity: 0.05 ng/mL
Sample Type: Human serum / 50 μL
Calibrator Range: 0.1–25.6 ng/mL
Total Assay Time: 75 minutes
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Note: When using the kit, the IFU version that is provided with the kit must be followed.

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Medical applications

Pregnenolone

Pregnenolone (3β-hydroxypregn-5-en-20-one) is the first steroid to be derived from cholesterol in the pathway of steroidogenesis, and it is the common precursor for all of the adrenal and gonadal steroids. Its production occurs in the mitochondrion by cleavage of the C-20 side chain of cholesterol by the P-450SCC enzyme. Once produced, pregnenolone may be utilized by two pathways of steroidogenesis. Pregnenolone may either be converted to 17-OH pregnenolone via the enzymatic action of 17α-hydroxylase or to progesterone via the enzymatic action of 3β-hydroxysteroid dehydrogenase.