DBC LIA
Reference: CAN-FT4-6040
Kit Type: Competitive LIA
Kit Size: 96-well break-apart microplate
Sensitivity: 1 pg/mL
Sample Type: Human serum / 25 μL
Calibrator Range: 2–80 pg/mL
Total Assay Time: 90 minutes
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Note: When using the kit, the IFU version that is provided with the kit must be followed.

  • CAN: 
  • EU:  
  • USA: For Research Use Only. Not For Use In Diagnostic Procedures.

Medical applications

Thyroxine-Free

Thyroxine (T4), the principal thyroid hormone, circulates in blood almost completely bound to carrier proteins. However, only the free (unbound) fraction of thyroxine is considered to be biologically active. The main carriers of thyroxine are thyroxine-binding globulin (TBG), pre-albumin and albumin. The measurement of free thyroxine (fT4) levels correlate better with the clinical status than total thyroxine levels.

The DBC free T4 assay is a one step competitive LIA system that is rapid and easy to perform compared to equilibrium dialysis and ultrafi ltration methods, which are cumbersome and time-consuming. This system employs a highly specifi c monoclonal antibody and a non-analog tracer that was proved experimentally to have no signifi cant binding to TBG and albumin.

In the euthyroid, normal population the free T4 concentration is 7–22 pg/mL. The level of free T4 is decreased in hypothyroidism while in thyrotoxic patients the level of free T4 is increased.

This assay is used at times with other thyroid tests for in vitro diagnostic purposes and for assessing patients who are receiving thyroid treatments (follow-up).