DBC is excited to announce the impending arrival of its NEW
Glucagon ELISA kit
DBC's Glucagon ELISA kit feartures:
  • A short incubation time
  • No shaking
  • Room temperature incubation
  • No Biotin conjugate
Make sure to check back for updates, or
contact us for more information
Glucagon ELISA kit
REF CAN-GLN-380
Performance
Sensitivity
LoB: 96 replicates of each lot of Cal A (4 lots in total) across 6 tests (3 kit lots, 2 tests for each kit lot) were run.
LoD: 96 replicates of each low-value sample (4 samples in total) across 6 tests (3 kit lots, 2 tests for each kit lot) were run.
LoQ: Lowest glucagon concentration that produces a sample CV% of less than 20%
Parameter DBC Glucagon, pg/mL
LoB 0.80
LoD 2.3
LoQ 5.3
Cross-Reactivity
Non-detectable Cross-reactivity for 21 cross-reactants (including Oxyntomodulin and Glicentin)
Interference
36 interferents tested
Linearity
CLSI EP06-Ed2, the kit is sufficiently linear up to a 1:10 dilution.
Precision
Repeatability ≤ 2.4%: used a nested components-of-variance design with 20 testing days, two tests per day, and two replicates per run for each sample (a 20 x 2 x 2 design, total n=80). Included five EDTA plasma samples and two kit controls.
Reproducibility ≤ 9.5%: used an experimental design model 3 x 5 x 5 (3 conditions x 5 testing days x 5 replicates per sample per day, total n=75). The study included five EDTA plasma samples and two kit controls.
Kit Accelerated Stability
At 37°C, stable for up to 11 days for all 3 kit lots.
In-use Stability
Up to 3 months before the expiration date.
Reference Range
Healthy, non-diabetic individuals
Results: 95% Range 7.1–66.3 pg/mL
Comparison
Correlation between DBC Glucagon ELISA and Mayo Glucagon Immunoassay:
r2 = 0.94 (r = 0.97), slope 0.94, significant commutability.
Plasma samples comparative analysis