Glucagon ELISA kit
- A short incubation time
- No shaking
- Room temperature incubation
- No Biotin conjugate
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LoB: 96 replicates of each lot of Cal A
(4 lots in total) across 6 tests (3 kit lots, 2 tests for each kit lot) were run.
LoD: 96 replicates of each low-value sample (4 samples in total) across 6 tests (3 kit lots, 2 tests for each kit lot) were run.
LoQ: Lowest glucagon concentration that produces a sample CV% of less than 20%
Parameter | DBC Glucagon, pg/mL |
LoB | 0.80 |
LoD | 2.3 |
LoQ | 5.3 |
Results: 95% Range 7.1–66.3 pg/mL
r2 = 0.94 (r = 0.97), slope 0.94, significant commutability.