Enzyme-linked immunosorbent assay (ELISA), also known as enzyme immunoassay (EIA), is one of the most clever and economical analytical technologies ever implemented in clinical diagnostics. Forty years after its invention, ELISA is used worldwide and produces millions of diagnostic results daily.
THE BASICS
Today, every biological fluid is used as a specimen in clinical diagnostics, but the most common bodily fluids are serum, plasma, saliva and urine. Each fluid, defined also as matrix, demands elaborate collection and preparation procedures which determine the accuracy of the analytical method. Improper sample preparation can lead to results very distant from the actual concentration of the analyte.
Careful planning and organization of the test is often underestimated, leading to costly mistakes or, even worse, to inadvertent inaccuracies. It is essential to fully study the test instructions for use (IFU), prior to the running the test and confirm that trained personnel, time and material resources are in place to run the test.
TROUBLESHOOTING

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