During pregnancy the determination of estriol is used for monitoring fetal well being. Therefore, during pregnancy the estriol level can detect abnormalities in the development of the fetus. As pregnancy progresses in time the concentration of Estriol also increases. The unconjugated estriol saliva determination is of great clinical importance to follow the progress of pregnancy since the level of salivary unconjugated estriol reflects the unconjugated estriol concentration in serum, which reﬂ ects the biologically active fraction.
The collection of saliva has advantages compared to urine and serum since it is a non-invasive procedure which can be easily collected at home and stored frozen.
Due to the low concentration of unconjugated estriol in saliva we have developed an assay system using a chemiluminescent immunoassay (LIA). The assay is very sensitive (0.03 ng/mL), precise and accurate along with a short incubation time of 75 minutes at RT° on a shaker.