3alpha-Diol G ELISA kit, REF: CAN-DG-460
Ref CAN-DG-460
Kit Type: Competitive ELISA
Kit Size: 96-well break-apart microplate
Sample Type: Human serum / 50 μL
Calibrator Range: 0.25–50 ng/mL
Total Assay Time: 45 minutes
Note: When using the kit, the IFU version that is provided with the kit must be followed.
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  • CAN: IVD
  • EU: IVD CE
  • USA: For Research Use Only. Not For Use In Diagnostic Procedures.
5α-Androstane 3α, 17β-diol Glucuronide (3α Diol G)

The 3α-Diol G ELISA is a competitive immunoassay. Competition occurs between 3α-Diol G present in calibrators, controls, specimen samples and an enzyme-labelled antigen (HRP conjugate) for a limited number of anti-3α-Diol G antibody binding sites on the microplate wells. After a washing step that removes unbound materials, the TMB substrate (enzyme substrate) is added which reacts with HRP to form a blue-coloured product that is inversely proportional to the amount of 3α-Diol G present. Following an incubation, the enzymatic reaction is terminated by the addition of the stopping solution, converting the colour from blue to yellow. The absorbance is measured on a microplate reader at 450 nm. A set of calibrators is used to plot a calibrator curve from which the amount of 3α-Diol G in specimen samples and controls can be directly read.

Medical applications