DBC Free Estriol Serum ELISA kit, kit image, silhouette of a pipette
Ref CAN-E-640
Kit Type: Competitive ELISA
Kit Size: 96-well break-apart microplate
Sample Type: Human serum / 10 μL
Calibrator Range: 0.05–30 ng/mL
Total Assay Time: 80 minutes
Note: When using the kit, the IFU version that is provided with the kit must be followed.
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  • CAN / EU: For research use only. Not for use in diagnostic procedures.
  • USA: IVD

The Free Estriol ELISA is a competitive immunoassay. Competition occurs between estriol present in calibrators, controls, specimen samples and an enzyme-labelled antigen (HRP conjugate) for a limited number of anti-estriol antibody binding sites on the microplate wells. After a washing step that removes unbound materials, the TMB substrate (enzyme substrate) is added which reacts with HRP to form a blue-coloured product that is inversely proportional to the amount of estriol. Following an incubation, the enzymatic reaction is terminated by the addition of the stopping solution, converting the colour from blue to yellow. The absorbance is measured on a microplate reader at 450 nm. A set of calibrators is used to plot a calibrator curve from which the amount of estriol in specimen samples and controls can be directly read.

Medical applications