The Instructions for Use (IFU) provided on this website are for informational purposes only. Always refer to the IFU included in the kit for the most current and accurate version.
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The Progesterone ELISA is a competitive immunoassay. Competition occurs between progesterone present in calibrators, controls, specimen samples and an enzyme-labelled antigen (HRP conjugate) for a limited number of anti-progesterone antibody binding sites on the microplate wells. After a washing step that removes unbound materials, the TMB substrate (enzyme substrate) is added which reacts with HRP to form a blue-coloured product that is inversely proportional to the amount of progesterone present. Following an incubation, the enzymatic reaction is terminated by the addition of the stopping solution, converting the colour from blue to yellow. The absorbance is measured on a microplate reader at 450 nm. A set of calibrators is used to plot a calibrator curve from which the amount of progesterone in specimen samples and controls can be directly read.
