Anti-TPO ELISA kit (CAN-ATP-4160)
Ref CAN-ATP-4160
Kit Type: Sandwich ELISA
Kit Size: 96-well break-apart microplate
Sample Type: Human serum
Total Assay Time: 80 minutes
The Instructions for Use (IFU) provided on this website are for informational purposes only. Always refer to the IFU included in the kit for the most current and accurate version.
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The Anti-TPO ELISA is a two-step capture or ‘sandwich’ type immunoassay. Thyroid peroxidase (TPO) antigen is immobilized onto the microplate. In the first incubation step, anti-TPO autoantibodies present in the specimen samples, calibrators and controls is bound to the immobilized antigen. Excess and unbound materials are removed by a washing step. In the second incubation step, anti-human-IgG HRP conjugate is added, which binds specifically to any autoantibodies that are bound to the immobilized antigen. Unbound HRP conjugate is removed by a washing step. Next, the TMB substrate (enzyme substrate) is added which reacts with HRP to form a blue coloured product that is directly proportional to the amount of anti-TPO autoantibodies present. The enzymatic reaction is terminated by the addition of the stopping solution, converting the colour from blue to yellow. The absorbance is measured on a microplate reader at 450 nm. A set of calibrators is used to plot a calibrator curve from which the amount of anti-TPO autoantibodies in specimen samples and controls can be directly read.