DBC Reverse Triiodothyronine (RT3) ELISA kit, kit image, silhouette of a pipette
Ref CAN-RT3-100
Kit Type: Competitive ELISA
Kit Size: 96-well break-apart microplate
Sample Type: Human serum or plasma / 25 μL
Calibrator Range: 0.02–2 ng/mL
Total Assay Time: 105 minutes
Note: When using the kit, the IFU version that is provided with the kit must be followed.
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  • CAN: IVD
  • EU: IVD CE
  • USA: For Research Use Only. Not For Use In Diagnostic Procedures.

The rT3 ELISA is a two-step competitive immunoassay. In the first incubation step, competition occurs between rT3 present in calibrators, controls, specimen samples and a biotin-labelled antigen (biotin conjugate) for a limited number of anti-rT3 antibody binding sites on the microplate wells.

Excess and unbound materials are removed by a washing step.

In the second incubation step, streptavidin-HRP (streptavidin HRP conjugate) is added, which binds specifically to any bound biotin conjugate. After a washing step that removes unbound materials, the TMB substrate (enzyme substrate) is added which reacts with HRP to form a blue-coloured product that is inversely proportional to the amount of rT3 present. Following an incubation, the enzymatic reaction is terminated by the addition of the stopping solution, converting the colour from blue to yellow. The absorbance is measured on a microplate reader at 450 nm. A set of calibrators is used to plot a calibrator curve from which the amount of rT3 in specimen samples and controls can be directly read.