DBC Free Testosterone ELISA kit, kit image, silhouette of a pipette
Ref CAN-FTE-260
Kit Type: Competitive ELISA
Kit Size: 96-well break-apart microplate
Sample Type: Human serum / 25 μL
Calibrator Range: 0.1–60 pg/mL
Total Assay Time: 75 minutes
Note: When using the kit, the IFU version that is provided with the kit must be followed.
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  • CAN: IVD
  • EU: IVD CE
  • USA: IVD

The Free Testosterone ELISA is a competitive immunoassay. Competition occurs between free testosterone present in calibrators, controls, specimen samples and an enzyme-labelled antigen (HRP conjugate) for a limited number of anti-free testosterone antibody binding sites on the microplate wells. After a washing step that removes unbound materials, the TMB substrate (enzyme substrate) is added which reacts with HRP to form a blue-coloured product that is inversely proportional to the amount of free testosterone present. Following an incubation, the enzymatic reaction is terminated by the addition of the stopping solution, converting the colour from blue to yellow. The absorbance is measured on a microplate reader at 450 nm. A set of calibrators is used to plot a calibrator curve from which the amount of free testosterone in specimen samples and controls can be directly read.

The DBC free testosterone kit utilizes a highly specific rabbit anti-testosterone polyclonal antibody at a low binding capacity (Keq x concentration) to keep minimum disturbances of the testosterone-protein equilibrium. The other components in the test system are also optimized in order to not alter the original free testosterone concentration.